Inoculation

Efficient novel technologies for production of Chips and Oil

All

INOCULATION TECHNOLOGY

OUD MIT 16 TECHNIQUE

AGARWIT 100 KIT

OUDZENE INJECTION

BIOSOCH-P METHOD

GARAMONE A10 KIT

OUD LB1 INJECTION

OUD MIT 18 TECHNIQUE

BIOZEN - AFC

AGARWIT 400 KIT

OUD MIT 20 TECHNIQUE

KRISTOZEN W-2 INJECTION

AGARWIT 500 KIT

BIOZEN - WMF

CHEMOSACH - P METHOD

OLETON B3 INJECTION

HPFCI - M10

INOCULATION AFTER CARE

BIO Sach-P

Bio Sach-P This method is used for the production of extraction grade. This method is best suitable to get yearly income same as any other plantation crops. Further cost of medicine as well labour is cheaper in compare to any other inoculation methods.

Selection of tree

Matured Agarwood tree aged over 7-8 years with circumference of 50 – 60 cm is chosen for adopting this method.

Inoculation Procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Peel the bark of the tree starting from the marked area and continue up to 1 ft circumference in the top.
  • About half the portion of the bark is peeled, leaving behind remaining portion of the bark to allow tree growth.
  • Paint the medicine for pealed area with the help of   brush.
  • Repeat painting on same area second time once after previous medicine is completely dried up.

Harvesting Procedure

  • The painted area can be chopped and extraction grade is collected once in a year time frame.
  • After harvesting again paint medicine to harvested area and get another harvest in one year
  • This method of painting and harvesting is continued for 4-5 years.
  • Later on the 4th year, on the opposite side of chopped area, drill 8mm hole and inject Oleton   B3 injection.
  • Oleton B3 injection is injected for all branches as well.
  • Finlay harvests the whole tree after 2 years of injection to get extraction grade material.     
  • After harvesting at two ft from ground level new 2 shoots is allowed grow to get second  Crop.

 

 

 

OUD MIT 16 TECHNIQUE

Oudmit-16 inoculation technology is best suitable for production of resinous chips. Our inoculation kit comes up

with a pack of 25 to 50 sticks and a bottle of medicine.

Selection of tree

Matured agarwood tree aged over 7-8 years with circumference above 60 cm is relevant for Oudmit-16 method of inoculation.

Inoculation Procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark from the starting point for the inoculation.
  • Drill the first hole for about 20 cm from the marked area. Continue drilling the hole up to 30cm circumference in the top.
  • Use 6.15mm bit for drilling. If the circumference of the tree is more than 20 inches drill the hole for it’s full length.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length. And depth of drilling should be half the length of the bit if the tree trunk is less than 12 inches.
  • Drilling direction should be towards side wise and it shouldn’t focus for the centre of the tree trunk. It should be eccentric with an angle of 45°.
  • If in case drilled towards centre of the trunk medicine will not distribute uniformly. Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Distance between holes should be 15cm in lateral direction and 17.5cm in the vertical direction.
  • Position of the hole should be alternative in Zig-Zag shape.
  • Immediately after drilling the holes, insert the inoculum sticks with the hammer.
  • Second round of inoculation is given after 6 months from the date of first inoculation on one side of the previous holes.
  • Third round of inoculation is recommended depending upon the response of the trees as per the observation from our technicians.
  • If it seems tree may need more medicine, the inoculation is done after 3-6 months of 2nd inoculation, to the other side of 2nd time inoculated hole.
  • On the top portion of the tree where circumference of tree is less than 30 cm  injection recommended approximately for every 30 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.

Harvesting of the Tree

  • After 36 months of inoculation resin will be deposited around inoculated place which will be then separated as Chips.
  • Depending on finical requirements of farmer, tree can be either harvested in single or multiple phases. 
  • If multiple phase harvesting is followed in the first phase harvest, only top portion of the tree is harvested leaving behind the main stem.     
  • Later on new shoots will develop on main stem which encourages further deposition of resin in the inoculated area.
  • Main stem will be harvested in 20-24 months which will further increase resin and the production of quality of the chips.
  • Harvesting is done after 2 feet from the ground level to allow regeneration of the tree.

 

 

agarwit 500 kit

Agarwit 500 inoculation is whole tree inoculation method. It is best suitable for production of extraction grade raw material for manufacturing of oil. Our inoculation kit comes up with a pack of 5 bags. Each bag is loaded with 1000 ml medicine. Kit includes required accessories for assembling and fixing medicine bags to the tree.

Selection of tree

Agar wood tree aged over 6-7 years with circumference of 50 cm and above is suitable for this method. Deepening on girth and height of the tree number of bags of medicine may go to vary. In general company recommends this method for small trees with average height of 20 ft.

Inoculation Procedure

  • Leave the tree trunk for about 100 cm from the ground level and mark for the first inoculation point.
  • Each bag of medicine comes up with a pipe which is divided in such a way that it can be fixed for 2 holes.
  • Drill 5.5 mm hole for fixing medicine pipe.
  • Drill first hole towards the centre of the trunk.
  • Second hole is pointed at about 6 inches in the lateral direction and 6 inches in the vertical direction from the first hole.
  • If tree circumference is more than 60 cm then you may need to 2 bags of medicine at one level.
  • In such case again drill a hole about 6 inches in later direction and 6 inches in the vertical direction and fix medicine kit.
  • Second level of inoculation is recommended at 360 cm from first inoculated point.
  • For the tree with circumference of 50 cm, fix the medicine in the second level with 2 bags of medicine.
  • If the tree circumference is 40 cm and then one bag of medicine can be fixed.
  • Drill about half the number of the holes towards centre and remaining half towards border of the tree trunk.
  • If in case all holes drilled towards centre of the trunk medicine will not distribute uniformly.
  • Drill the hole 45 degree towards downside of the tree to enable easy flow of medicine.
  • Immediately after drilling the hole fix it with the inducing equipment and release the liquid.
  • The medicine bag should be placed in about 3 feet from the inserted area through discharge pipe in order to make flow of the medicine by gravitational force.
  • Inoculum will be observed within 4-5 days depending upon the weather condition.
  • Second level of inoculation is recommended at 360 cm from first inoculated point.
  • For the tree with circumference of 50 cm, fix the medicine in the second level with 2 bags of medicine.
  • If the tree circumference is 40 cm and then one bag of medicine can be fixed.
  • Drill about half the number of the holes towards centre and remaining half towards border of the tree trunk.
  • If in case all holes drilled towards centre of the trunk medicine will not distribute uniformly.
  • Drill the hole 45 degree towards downside of the tree to enable easy flow of medicine.
  • Immediately after drilling the hole fix it with the inducing equipment and release the liquid.
  • The medicine bag should be placed in about 3 feet from the inserted area through discharge pipe in order to make flow of the medicine by gravitational force.
  • Inoculum will be observed within 4-5 days depending upon the weather condition.

Booster dosage

  • Normally this method is adopted one time only. If the dosage given is not sufficient then booster doze will be recommended as per physical observation of the tree by our technical team.
  • Booster dosage can be given in the time frame of 6 months for the top portion of the tree where inoculation was not done earlier.

Harvesting of the Tree

  • After inoculation resin will be deposited all along the tree trunk especially in the centre where inoculum medicine is circulated.
  • As Agarwit – 500 is a whole tree inoculation method complete tree will be harvested in the time frame of 12 months. 
  • After harvesting resinous wood portions are segregated and used for the distillation of the oil.

biozen afc

Biozen AFC is used for the production of  Chips or Extraction grade material depending on when tree is harvested. It’s a microbial consortium. Inoculation medicine comes up with a two pack powder and a bottle of liquid. All the ingredients used in the manufacturing of the Biozen AFC are from natural source. Formation of Agarwood in this method is less, however good quality of Agarwood is expected from the method.  Formation is more or less like natural phenomena with good quality resinous wood production. 

Agarwood tree of any age group over 6 years and even more than 15 years are eligible for adopting this method.  The production formula and ingredients are cost effective hence  this is  affordable method and particularly suitable for large sized trees.

Inoculation Procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Drill the first hole for about 15 cm from the marked area. Continue drilling the hole up to 30cm circumference in the top.
  • Use 8 mm bit for drilling. If the circumference of the tree is more than 16 inches drill the hole for it’s full length.
  • If girth is less than 16 inches drill the bit for about 2/3rd of it’s length. And depth of drilling should be half the length of the bit if the tree trunk is less than 10 inches.
  • Drilling direction should be towards side wise and it shouldn’t focus for the centre of the tree trunk.  It should be eccentric with an angle of 45°.

Inoculation Procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Drill the first hole for about 15 cm from the marked area. Continue drilling the hole up to 30cm circumference in the top.
  • Use 8 mm bit for drilling. If the circumference of the tree is more than 16 inches drill the hole for it’s full length.
  • If girth is less than 16 inches drill the bit for about 2/3rd of it’s length. And depth of drilling should be half the length of the bit if the tree trunk is less than 10 inches.
  • Drilling direction should be towards side wise and it shouldn’t focus for the centre of the tree trunk.  It should be eccentric with an angle of 45°.
  • Distance between holes should be 12 cm in lateral direction and 15 cm in the vertical direction.
  • Position of the hole should be in triangle shape.
  • Mix both the packs of powder which includes different type of fungal cultures with given liquid which act as a primary food for fungus.
  • Immediately after drilling the holes, inject mixture of Biozen AFC through specially designed bottle.
  • Close the hole with wax  in order to stop entry of water in the rainy season.
  • On the top portion of the tree where circumference of tree is less than 20 cm injection recommended approximately for every 15 cm distance in the zig-zag manner.
  • Inject the liquid for the branches and twigs as well in order to increase the production.
agarwit500

Harvesting of the Tree

  • After inoculation resin will be deposited around inoculated place which will be then separated as Chips.
  • Trees can be harvested after 40 months of inoculation at about 2 ft from the ground level.
  • Trees are recommended for harvesting at about two ft from the ground level and two new suckers are allowed to grow to get next crop.

 

oudmit 18 technique

For production of Chips Oudmit-18 inoculation technology is well suitable.

Selection of tree

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Drill the first hole for about 20 cm from the marked area. Continue drilling the hole up to 30cm circumference in the top.
  • Use 6.15mm bit for drilling. If the circumference of the tree is more than 20 inches drill the hole for it’s full length.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length. And depth of drilling should be half the length of the bit if the tree trunk is less than 12 inches.
  • Drilling direction should be towards side wise and it shouldn’t focus for the centre of the tree trunk.  It should be eccentric with an angle of 45°.
  • If in case drilled towards centre of the trunk medicine will not distribute uniformly. Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Distance between holes should be 15 cm in lateral direction and 15 cm in the vertical direction.
  • Position of the hole should be square shape.
  • Immediately after drilling the holes, insert the inoculum sticks with the hammer.
  • Second round of inoculation is given after 6 months from the date of first inoculation in-between previous holes.
  • On the top portion of the tree where circumference of tree is less than 30 cm injection recommended approximately for every 30 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.

Harvesting of the Tree

  • After 36 months of inoculation resin will be deposited around inoculated place which will be then separated as Chips.
  • Depending on finical requirements of farmer, tree can be either harvested in single or multiple phases. 
  • If multiple phase harvesting is followed in the first phase harvest, only top portion of the tree is harvested leaving behind the main stem.    
  • Later on new shoots will develop on main stem which encourages further deposition of resin in the inoculated area.
  • Main stem will be harvested in 20-24 months which will further increase resin and the production of quality of the chips.
  • Harvesting is done after 2 feet from the ground level to allow regeneration of the tree.

OUD LB1 INJECTION

Oud LB-1 this method is used for the production of chips. Our inoculation medicine kit comes up with a pack four bottles which will be enough for a medium sized tree.

Selection of tree

Matured Agarwood tree aged over 7-8 years with circumference above 60 cm is suitable for Oud Lb1 method of inoculation. In general company will recommend medium to large size trees for adaptation of this method. 

Inoculation Procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Drill the first hole for about 20 cm from the marked area. Continue drilling the hole up to 30cm circumference in the top.
  • Use 6 mm bit for drilling. If the circumference of the tree is more than 20 inches drill the hole for it’s full length.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length. And depth of drilling should be half the length of the bit if the tree trunk is less than 12 inches.
  • Drilling direction should be towards side wise and it shouldn’t focus for the centre of the tree trunk.  It should be eccentric with an angle of 45°.
  • If in case drilled towards centre of the trunk medicine will not distribute uniformly. Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Distance between holes should be 15 cm in lateral direction and 20 cm in the vertical direction.

Inoculation Procedure

  • Position of the hole should be in triangle shape.
  • Immediately after drilling the holes, inject Oud Lb1 injection through injection syringe.
  • Close the hole with hard wood stick or silica gel in order to stop entry of water in the rainy season.
  • On the top portion of the tree where circumference of tree is less than 30 cm  injection recommended approximately for every 25 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.

 

 

Harvesting of the Tree

  • After inoculation resin will be deposited around inoculated place which will be then separated as Chips.
  • Trees can be harvested after 36 months of inoculation at about 2 ft from the ground level.             
  • Trees are recommended for harvesting at about two ft from the ground level and two new suckers are allowed to grow to get next crop.

GARAMONE A10 KIT

Garamone A10  inoculation technology is best suitable for production of resinous chips. Our inoculation kit comes up with a pack of 50 bottles of medicine which will be sufficient dose for a medium sized tree. Each bottle loaded with 10 ml of food grade medicine. Bottle lid has a suitable shape which can be easily fixed to tree trunk.

Selection of tree

Matured Agarwood tree aged over 7-8 years with circumference above 60 cm is suitable for inoculation of Garamone A10 method of inoculation. Under normal circumstances company will recommend medium sized trees for adaptation of this method. 

Inoculation Procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation
  • Drill the first hole for about 25 cm from the marked area.
  • Coil the rope around tree up to 65% of the tree height.
  • Use 5 mm bit for drilling the hole.
  • Drill the hole up to 65 % of the tree high in the first dosage of medicine.
  • In order to mark drilling hole properly use the plastic rope which is marked for every 20 cm.
  • Maintain the distance between two coils for about 20 cm.
  • Drill the bit for the full length and care should be taken that it should be at-least two inch less than radius of the tree.
  • Drill the hole up to 65 % of the tree high in the first dosage of medicine.
  • In order to mark drilling hole properly use the plastic rope which is marked for every 20 cm.
  • Maintain the distance between two coils for about 20 cm.
  • Drill the bit for the full length and care should be taken that it should be at-least two inch less than radius of the tree.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length.
  • And depth of drilling should be quarter the length of the bit if the tree trunk is less than 10 inches.
  • Drilling direction shouldn’t focus for the centre of the tree trunk. Is should be towards side wise and eccentric with an angle of 45⁰.
  • If in case drilled towards centre Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • When drilled 2/3 or half of the length, put only more or less half of the medicine in the bottle.
  • Immediately after drilling, fix the medicine bottle directly to the tree trunk.
  • Liquid will be absorbed in to the tree trunk within 12 hours depending on the weather condition.
  • After 6 month of first round of inoculation, second round of inoculation is recommended for remaining 35 % of top portion of the tree.
  • Injection also recommended for the branches and twigs in order to increase the production.
  • Close the hole with hard wood stick or silica gel in order to stop entry of water in the rainy season.

oudzene injection

Oudzene injection is used for the production of chips. Our inoculation medicine kit comes up with a pack of three bottles which will be enough for a small to medium sized tree. The method is best suitable for the developing small size and pocket shaped chips.

Selection of tree

  • Drill the hole up to 65 % of the tree high in the first dosage of medicine.
  • In order to mark drilling hole properly use the plastic rope which is marked for every 20 cm.
  • Maintain the distance between two coils for about 20 cm.
  • Drill the bit for the full length and care should be taken that it should be at-least two inch less than radius of the tree.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length.

Inoculation Procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark from the starting point for the inoculation.
  • Drill the first hole for about 15 cm from the marked area. Continue drilling the hole up to 30cm circumference in the top.
  • Use 4 mm bit for drilling. If the circumference of the tree is more than 16 inches drill the hole for it’s full length.
  • If girth is less than 16 inches drill the bit for about 2/3rd of it’s length. And depth of drilling should be half the length of the bit if the tree trunk is less than 10 inches.
  • Drilling direction should be towards side wise and it shouldn’t focus for the centre of the tree trunk.  It should be eccentric with an angle of 45°.
  • If in case drilled towards centre of the trunk medicine will not distribute uniformly. Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Distance between holes should be 15 cm in lateral direction and 20 cm in the vertical direction.
  • Position of the hole should be in triangle shape.
  • Immediately after drilling the holes, inject Oudzen injection through injection syringe.
  • Close the hole with hard wood stick or silica gel in order to stop entry of water in the rainy season.
  • On the top portion of the tree where circumference of tree is less than 20 cm injection recommended approximately for every 20 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.

Harvesting Procedure

  • After inoculation resin will be deposited around inoculated place which will be then separated as Chips.
  • Trees can be harvested after 36 months of inoculation at about 2 ft from the ground level.          
  • Trees are recommended for harvesting at about two ft from the ground level and two new suckers are allowed to grow to get next crop. 

Agarwit 100 kit

AGARWIT 100 method is a whole tree inoculation technique. It is a best suitable for production of extraction grade raw material for manufacturing of oil. Our inoculation kit comes up with a pack of 10 bottles of medicine along with required accessories for assembling and fixing medicine bottles to the tree.

Selection of tree

Matured agar wood tree aged over 7-8 years with circumference of 50 cm and above is suitable for this method. For large size trees where in tree height is over 35 ft this method is installed for the top portion of the tree along with any other compatible method for the bottom. 

Procedure

  • Leave the tree trunk for about 1.5 feet from the ground level and mark for the starting point for the inoculation.
  • Drilling can be continued upto 20cm circumference of the tree trunk in the top portion.
  • Use 12 mm bit for drilling for whole tree and even at the top portion of the tree as well.
  • Drilling direction should be towards side wise and it shouldn’t focus for the centre of the tree trunk.  It should be eccentric with an angle of 45°.
  • If in case drilled towards centre of the trunk medicine will not distribute uniformly. Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Distance between the holes should be 60 cm in the vertical direction and 15 cm in lateral direction.
  • Position of the holes should be in the zig-zag direction.
  • Depending on the girth of the tree distance between holes can be increased up to 75cm.
  • Drill the hole 45 degree towards downside of the tree.
  • Immediately after drilling the hole fix it with the inducing equipment and release the liquid.
  • The medicine bottle should be placed in about 2 feet from the inserted area through discharge pipe in order to flow the medicine by gravitational force.                                     
  • Inoculum will be observed within 5-7 days depending upon the weather condition.

On the top of the tree

  • If the height of the tree is above 35 feet this method can be adopted only for the top portion of the tree or along with other method at the bottom.
  • For branches drill the hole with 12mm bit in the zig zag manner for every 3-4 feet distance deepening on girth.
  • Around half dosage of medicine is recommended for branches.

Booster Dosage

  • Normally this method is adopted only one time. If the dosage given is not sufficient then boosted doze will be recommended as per physical observation of the tree by our technical team.
  • Booster dosage can also be given in the empty spaces in-between inoculated spots in order to increase the yield.
  • Injections also recommended for the branches and twigs in order to increase the production.

Harvesting of the tree

After inoculation resin will be deposited all along the portion of the tree where ever inoculum medicine is circulated. As Agarwit -100 is a whole tree inoculation method complete tree can be harvested in the time frame of 18-24 months.  After harvesting resinous wood portions are segregated and used for the distillation of the oil.

HPFCI - M10

HPFCI- M10 method is useful for production of extraction grade material.  Our inoculation medicine kit comes up with a pack 2 bottles of medicine and injectors for inoculation. Method involves injecting medicine liquid with specially designed injectors with pressure and enable quick flow of medicine into tree trunk. 

Selection of tree

Agarwood tree aged about 8 to 10 years with circumference above 50 cm – 60 cm is ideal for inoculation of in this method. Under normal circumstances company will recommend medium sized trees with tree height less than 25 ft for adaptation of this method. 

  • Drill the first hole for about 30 cm from the ground level.
  • And next level of injection will be given for every 75 cm height.
  • Continue drilling the hole up to 30 cm circumference in the top.
  • Use 5 mm bit for drilling the hole.
  • Distance between holes should be 5 inch in the lateral direction and 7 inch in the vertical direction.
  • Continue drilling the hole spirally around the trunk and then move to next level at 75 cm.
  • If the circumference of the tree is more than 20 inches drill the hole for it’s full length. And Load about 15 ml of medicine to injector and realise it to tree trunk.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length. And Load about 10 ml of medicine to injector and realise it to tree trunk.
  • And depth of drilling should be half the length of the bit if the tree trunk is less than 10 inches. And Load about 10 ml of medicine to injector and realise it to tree trunk.
  • Medicine will be absorbed within 10 minutes. Takeout the injector and fix hard wood stick.
  • Drilling direction shouldn’t focus for the centre of the tree trunk. Is should be towards side wise and eccentric with an angle of 45⁰.
  • If in case drilled towards centre of the trunk medicine will not distribute uniformly. Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • In the first round of inoculation, do it for only half of the tree from the ground level.
  • And top half portion of tree, do the inoculation after 6 month of first round of inoculation.
  • In normal case booster dosage is not required and only these two round of inoculation is enough.
  • If the dosage given is not sufficient then boosted doze will be recommended as per physical observation of the tree by our technical team.

Harvesting of the tree

  • After inoculation resin will be deposited all along the portion of the tree where ever inoculum medicine is circulated.
  • HPFCI- M10 method is a multiple place and whole tree inoculation method, therefore whole tree can be harvested at ground level.
  • Harvesting duration will be on 24 months from the date of inoculation.
  • After harvesting resinous wood portions are segregated and used for the distillation of the oil.
   

agarwit - 400 kit

Agarwit 400 inoculation system is whole tree inoculation method. It is a best suitable for production of extraction grade raw material for manufacturing of oil. Our inoculation kit comes up with a pack of 16 bottles of medicine along with required accessories for assembling and fixing medicine bottles to the tree.

Selection of tree

Matured agar wood tree aged over 7-8 years with circumference of 50 cm and above is suitable for this method. For large size trees where in tree height is over 35 ft this method is installed for the top portion of the tree along with any other compatible method for the bottom. 

Inoculation Procedure

  • Leave the tree trunk for about 80 cm from the ground level and mark for the first inoculation point.
  • Second level of inoculation is recommended at 160 cm from first inoculated point.
  • For the tree with circumference of 60 cm drill two hole apposite to each other.
  • Drilling can be continued up to 30 cm circumference of the tree trunk in the top portion.
  • And at it is recommend to fix only one hole when tree circumference lesser than 45 cm.
  • Use 16 mm bit for drilling the tree up to 60 cm circumference about 1/3 of the tree portion.
  • Use 12 mm bit for middle portion of the tree about 2/3 of the area.
  • 1/3 of the top portion of the tree are not required to inoculate.

 

  • Drill about half the number of the holes towards centre and remaining half towards border of the tree trunk.
  • If in case all holes drilled towards centre of the trunk medicine will not distribute uniformly.
  • Tree trunk may damage due to over dose in the centre and create a hallow trunk if all the holes drilled towards centre.
  • Position of the holes should be in the zig-zag direction.
  • Depending on the girth of the tree distance between holes can be increased.
  • Drill the hole 45 degree towards downside of the tree to enable easy flow of medicine.
  • Immediately after drilling the hole fix it with the inducing equipment and release the liquid.
  • The medicine bottle should be placed in about 2 feet from the inserted area through discharge pipe in order to flow the medicine by gravitational force.
  • Inoculum will be observed within 10 days depending upon the weather condition.
 

Booster Dosage

  • Normally this method is adopted one time only. If the dosage given is not sufficient then boosted doze will be recommended as per physical observation of the tree by our technical team.
  • Booster dosage can be given in the time frame of 6 to 12 months for the 1/3rd portion of top portion of the tree where inoculation was not done earlier.

Harvesting of the tree

After inoculation resin will be deposited all along the portion of the tree where ever inoculum medicine is circulated. As Agarwit – 400 is a whole tree inoculation method complete tree can be harvested in the time frame of 24 months.  After harvesting resinous wood portions are segregated and used for the distillation of the oil.

chemosach - p method

Chemosach- P method is used for the production of extraction grade material. This method is best suitable to get yearly income same as any other plantation crops. Further cost of medicine is cheaper in compare to any other inoculation methods. It’s quite easy to do and trained workers are not required for the application of this technique.

Matured Agarwood tree aged over 6 years and girth over 50 cm can be chosen for the adaptation of this method. Even it is recommended for larger trees of any age and girth.

Inoculation Procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Peel the bark of the tree starting from the marked area and continue up to 1 ft circumference in the top.
  • About half the portion of the bark is peeled, leaving behind remaining portion of the bark to allow tree growth.
  • Paint the medicine for pealed area with the help of brush.
  • Repeat painting on same area second time once after previous medicine is completely dried up.

Harvesting of the tree

  • The painted area can be chopped and extraction grade is collected once in a year time frame.
  • After harvesting again paint medicine to harvested area and get another harvest in one year.
  • This method of painting and harvesting is continued for 4-5 years.
  • Later on the 4th year, on the opposite side of chopped area, drill 8mm hole and inject Kristhozen W2 injection.
  • Kristhozen W2 injection can be injected for all over the branches as well.
  • Finlay harvests the whole tree after 2 years of injection to get extraction grade material.
  • After harvesting at two ft from ground level new 2 shoots is allowed grow to get second crop.

Kristozen w-2 injection

Kristozen W-2 injection is used for the production of extraction grade material. Our inoculation medicine kit comes up with a pack 5 bottles which will be enough for a medium sized tree.

Selection of the tree

Matured Agarwood tree aged over 7-8 years with circumference above 60 cm is suitable for inoculation of Kristozen method of injection. Under normal circumstances company will recommend medium sized trees for adaptation of this method.

Inoculation procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Drill the first hole for about 30 cm from the marked area. Continue drilling the hole up to 30 cm circumference in the top.
  • Use 6 mm bit for drilling. If the circumference of the tree is more than 20 inches drill the hole for it’s full length.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length. And depth of drilling should be half the length of the bit if the tree trunk is less than 10 inches.
  • Drilling direction shouldn’t focus for the centre of the tree trunk. Is should be towards side wise and eccentric with an angle of 45⁰.
  • If in case drilled towards centre of the trunk medicine will not distribute uniformly. Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Distance between holes will be 17 cm in lateral direction and 60 cm in the vertical direction.
  • Position of the hole should be in rectangular shape.
  • Immediately after drilling  the holes, inject Kristhozen injection by syringe.
  • Second round of inoculation is given after 6 months from the date of first inoculation in-between the previous holes.         
  • On the top portion of the tree where circumference of tree is less than 30 cm  injection recommended approximately for every 40 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.
  • Only half dosage of medicine is recommend for branches.
  • Close the hole with hard wood stick or silica gel in order to stop entry of water in the rainy season.

Booster Dosage

  • In normal case booster dosage of inoculation is not required.
  • If the dosage given is not sufficient then boosted doze will be recommended as per physical observation of the tree by our technical team.
  • Booster dosage is recommended only for top half potion of the tree only if tree hight is over 30 ft.
  • Booster dosage of half the quantity of medicine is given in the empty spaces in-between inoculated spots in order to increase the yield.
  • Use 6 mm drill bit for drilling the hole.
  • Injections can also be recommended for the branches and twigs in order to increase the production.

Harvesting of the tree

  • After inoculation resin will be deposited all along the portion of the tree where ever inoculum medicine is circulated.
  • As Kristhozen injection is a multiple place and whole tree inoculation method, therefore whole tree can be harvested in the time frame of 24 months.
  • After harvesting resinous wood portions are segregated and used for the distillation of the oil.
  • Harvesting is done from 2 feet from the ground level to allow regeneration of the tree.

Biozen wmf

Biozen WMF  inoculation technology is suitable for production of both Chips and Extraction grade.  Our inoculation kit comes up with a pack of 100 bottles of medicine which will be sufficient dose for a medium sized tree. Each bottle loaded with 5 ml of food grade medicine. Bottle lid has a designed in the suitable shape which can be directly fixed to the tree trunk.

Selection of the tree

Agarwood tree with good amount of leaves and aged over 7-10 years with circumference above 60 cm is suitable for inoculation of Biozen WMF method of inoculation. Under normal circumstances company will recommend medium sized trees for adaptation of this method. 

Inoculation procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Drill the first hole for about 25 cm from the marked area.
  • Use 3 mm bit for drilling the hole.
  • Distance between holes should be 15 cm in the lateral direction and 20 cm in the vertical direction.
  • Drilling the hole in the diamond shape so that there is good distance between the hole in the vertical direction.
  • Continue drilling the hole up to 30 cm circumference in the top.
  • Drill the hole up to 65 % of the tree high in the first dosage of medicine.
  • Drill the bit for the full length and care should be taken that it should be at-least two inch less than radius of the tree.
  • Drilling direction shouldn’t focus for the centre of the tree trunk. Is should be towards side wise and eccentric with an angle of 45⁰.
  • If in case drilled towards centre Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Immediately after drilling, fix the medicine bottle directly to the tree trunk.
  • Liquid will be absorbed in to the tree trunk within 24 hours depending on the weather condition.
  • After 6 month of first round of inoculation, second round of inoculation is recommended for remaining 35 % of top portion of the tree.
  • Close the hole with hard wood stick or silica gel in order to stop entry of water in the rainy season.

Harvesting of the tree

  • After inoculation resin will be deposited around inoculated place which will be then separated as Chips or extraction grade.
  • Tree can be harvested in 24 months for production of extraction grade material.
  • For production of Chips trees can be harvested after 36 months of inoculation.          
  • Harvesting is recommended at about two ft from the ground level and two new suckers are allowed to grow to get next crop.

Oleton b3 injection

Oleton B3 injection is used for the production of extraction grade material. Our injection kit comes up with a pack four bottles which will be enough for a medium sized tree.

Selection of the tree

Matured Agarwood tree aged over 9-10 years with circumference above 60 cm is suitable for inoculation of Oleton B3 method of injection. Under normal circumstances company will recommend medium to large size trees for adaptation of this method.

Inoculation procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Drill the first hole for about 25 cm from the marked area. Continue drilling the hole up to 30cm circumference in the top.
  • Use 8 mm bit for drilling. If the circumference of the tree is more than 20 inches drill the hole for it’s full length.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length. And depth of drilling should be quater the length of the bit if the tree trunk is less than 12 inches.
  • Drilling direction shouldn’t focus for the centre of the tree trunk. Is should be towards side wise and eccentric with an angle of 45⁰.
  • If in case drilled towards centre of the trunk medicine will not distribute uniformly. Tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Distance between holes will be 15 cm in lateral direction and 40 cm in the vertical direction.
  • Position of the hole should be in square shape.
  • Immediately after drilling  the holes, inject oleton B-3 injection by syringe.
  • Second round of inoculation is given after 6 months from the date of first inoculation in-between the previous holes.         
  • On the top portion of the tree where circumference of tree is less than 30 cm  injection recommended approximately for every 25 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.
  • Only half dosage of medicine is recommend for branches.
  • Close the hole with hard wood stick or silica gel in order to stop entry of water in the rainy season.

Booster Dosage

  • Position of the hole should be in square shape.
  • Immediately after drilling  the holes, inject oleton B-3 injection by syringe.
  • Second round of inoculation is given after 6 months from the date of first inoculation in-between the previous holes.         
  • On the top portion of the tree where circumference of tree is less than 30 cm  injection recommended approximately for every 25 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.
  • Only half dosage of medicine is recommend for branches.
  • Close the hole with hard wood stick or silica gel in order to stop entry of water in the rainy season.

Harvesting of the tree

  • Position of the hole should be in square shape.
  • Immediately after drilling  the holes, inject oleton B-3 injection by syringe.
  • Second round of inoculation is given after 6 months from the date of first inoculation in-between the previous holes.         
  • On the top portion of the tree where circumference of tree is less than 30 cm  injection recommended approximately for every 25 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.
  • Only half dosage of medicine is recommend for branches.
  • Close the hole with hard wood stick or silica gel in order to stop entry of water in the rainy season.

INOCULATION TECHNOLOGY

In commercial Agarwood cultivation, it is possible to yield Agarwood even from 7- to 8-year-old (sometimes even younger) trees by drilling artificial holes in their trunks and infecting them. The main principle of all these processes is to drill holes in the tree trunk to inoculate a mixture of chemicals, organic compounds, or microorganisms.

Sixteen different technologies for inoculation have been developed at Vandurgi. Some of the techniques are developed in-house with a comprehensive technical knowhow of artificial inoculation. And few technologies are also developed in fruitful collaborations with leading international institutions. Such patented technologies are currently adopted in association with overseas partners at our plantations.

Based on requirement, the company identifies mature tress to be inoculated each year by discussing with farmers who wish to sell their produce. The company has well trained inoculation team, which works on all aspects right from identifying trees, inoculating them on proper time, carry out booster inoculation if required and harvesting till transporting trees to primary processing centre. Inoculation charges are reasonable and it is on cost to cost basis for its member planters of Vanadurgi.

Further, the company has conducted research that has led to an inoculation technology which produces less volume but better quality resinous wood. It is an efficient method that resembles the natural phenomena where simple and cost effective steps are adopted. The results so far have shown good quality resinous wood, similar to that of wild agarwood in terms of chemical constituents and the essential oil content, with a strong fragrance, ideal texture and desirable black resin. Inoculation has been started at Vanadurgi since 2014 although research is going since few years back. Further, during the year 2015 onwards new technologies has been added and commercial inoculation has been going on in in the farmer’s field. Currently inoculation has be implemented in all the growing Agarwood states of India by Vanadurgi.

Our team will inspect the trees and provide advice on identifying the right trees that are ready for harvesting by examining the infection rate. The age or size of the tree trunk does not decide the harvest time of commercially grown Agarwood. Trees can be harvested only when the formation of resinous wood is complete. When Agarwood is grown as a commercial crop, harvesting has to be done at a specified time for certain trees, making it a constant operation. Harvesting is done based on the method of inoculation followed for that particular tree. In general, trees which are inoculated for oil extraction will be harvested after 12 to 24 months. If trees are inoculated for chips, the harvesting time will be after 3 to 4 years, as longer the time before harvest, the better the quality.

INOCULATION After Care

Company is providing all required supports to farmers to maximise the yield and there by increase revenue.  During inoculation, growers must personally attend and share the necessary contents with the inoculation staff to ensure the inoculation is done in the right way. Providing growers full information about inoculation, pre- inoculation and post inoculation management is our priority. We recommend farmers to participate in the inoculation workshops which will be regularly held at the grower’s gardens.

Leaves work as kitchens for the production of resin and hence it’s important to maintain the lateral shoots and leaves. Further, resin formation is better in places near the leaves. Therefore, practising proper pruning system will help to grow the leaves all around the tree and thereby increase the production of Agarwood. Pruning should not be done after inoculation so that tree may have maximum leaves and thereby assisting the production of Agarwood.

During inoculation, the bark of the tree is holed. There are several wounds on the stem, especially when bamboo stick techniques are used. Also, when surface inoculation technique is practised, there is a large wounded area on one side of the bark. Such areas will create ready sites for fungal infections. However, if there is a sufficient dry period, then the wound sites will heal. To avoid infections, inoculate the trees after the rainy season, or in the summer. There are less chances of bark fungal infections when trees are inoculated with the whole-tree inoculation techniques as only a few inoculation holes are done on the trees. Inoculation reduces the immunity power of trees and therefore these trees are more susceptible for fungal bark infections.

If the Agarwood plantation is developed in moderate rain fall areas there is no necessity of fungicide spraying.  In heavy rain fall plantations, the company recommends the spraying of contact fungicides to prevent bark fungal infections. Bordeaux mixture is showing good results in the field. About 1 to 1.5% Bordeaux mixture spray in about 2 to 5 litres of water per tree, twice a year, at the commencement of the rainy season, and mid-rainy season, is recommended. If fungus has already destroyed parts of a tree, you can prevent it from spreading to the rest of the bark with fungicides. After the initial application to coat the surface of the tree, keep up the barrier with follow-up applications, especially when you spot new growth that needs protection. Also, keep in mind that fungicides are effective against diseases only for a limited time. For example, Bordeaux mixture is effective only for 40 to 50 days.

Farmers need to often observe the Inoculated trees. Due to medication, the inoculation trees get stress. There is no problem if the trees are in shade, but in the area where there is sunny and there is possibility of falling leaves due to the stress. At this time farmer may need have to give 1-2 times of water to the trees. There will be possibility of having stress to the cavity crop trees. Trees may die if there is more stress. Water is given for the trees if the harvesting is done in the summer.

Where there is gusty wind trees fall due it. During this time inoculation is done only for over half top of the tree about 20 – 25 feet hight. The remaining bottom half of the tree is done after top half portion is harvested. The Inoculation like Agar wit 100 Agar wit 400 etc., is not advisable to do for where the inoculation method is done to the whole tree. When it spreads to the full tree, there will be a large injury to the tree. At this time trees are likely to fall. Growers should look out to it.

Harvesting at the right time for inoculation trees can be possible of earning high income. Trees respond to inoculation, harvesting only after observing the production of agar. Factors such as the age of the tree or the size of the tree trunk cannot decide the harvest time of commercially grown Agarwood. Agarwood trees can be harvested only when the production of the aromatic trunk is complete, and the tree starts drying up. Generally, when Agarwood is grown as a commercial crop, harvesting has to be done at a specified time for certain trees, making it a constant operation.  March to May is the right time for harvesting, and within the commencement of the rainy season, harvested trees will produce new shoots. Care should be taken to see that harvested trees do not die due to stress. And, if required, it’s recommended to provide timely irrigation.

When trees are inoculated, we recommend leaving 2 ft from the ground level. Cutting the tree at this height will encourage better growth of new shoots as compared to harvesting them at ground level. Within two months of harvesting, new shoots start growing from the harvested area. By removing the weak shoots, only two good shoots. Select the shoots that are in the opposite direction so that both will grow without competing for sunlight, and the wound around the harvested area will also heal well. New growth emerges, and after a few years, the coppiced tree is harvested, and the cycle begins anew. Coppicing maintains the trees at a juvenile stage, and a regularly coppiced tree will never die of old age.

The staff of the company will provide the necessary information from time to time for the post inoculation management of the tree. If something is troubling you should call to company and get the solution and implement in the field.

OUD MIT 20 TECHNIQUE

Oudmit-20 inoculation technology is best suitable for production of resinous chips. Our inoculation kit comes up with a 4 packs wherein each contains of 25 -50 sticks. Kit also includes a bottle of medicine which is sufficient for a medium sized tree.

Selection  of the tree

Matured agarwood tree aged over 8 -10 years with circumference above 60 cm and above is suitable for Oudmit-20 method of inoculation. This method is also recommended for large sized trees which are aged over 15 years or more.

Inoculation procedure

  • Leave the tree trunk for about 2 feet from the ground level and mark for the starting point for the inoculation.
  • Drill the first hole for about 20 cm from the marked area. Continue drilling up to half the height of the tree.
  • Use 5.5 mm bit for drilling. If the circumference of the tree is more than 20 inches drill the hole for it’s full length.
  • If girth is less than 20 inches drill the bit for about 2/3rd of it’s length.
  • Drilling hole can be done as much as 30cm circumference in the top, but for only half the length of drill bit.
  • Drilling direction should be towards side wise and it shouldn’t focus for the centre of the tree trunk.  It should be eccentric with an angle of 45⁰.
  • If in case all holes drilled towards centre, tree trunk may damage due to over dose in the centre and create a hallow trunk.
  • Distance between holes should be 12 cm in lateral direction and 15 cm in the vertical direction.
  • Position of the hole should be alternative in Zig-Zag shape.
  • Immediately after drilling  the holes, insert the inoculum sticks with the help of a hammer.
  • Second round of inoculation is given after 3 months from the date  of first inoculation on the top of the half portion of the tree.
  • Third round of inoculation is given after 3 months from the date  of second inoculation for bottom half portion of the tree.
  • Fourth round of inoculation is given after 3 months from the date  of third round of inoculation for top half portion of the tree.
  • Further inoculation is recommended only if necessary depending upon the response of the trees as per observation from our technicians.
  • While doing inoculation, at first step drilling, direction of drilling need to be towards the centre.
  • For the second step it’s should be towards right side of the tree. And drilling is towards lift side in the 3rd step.
  • This is multidirectional drilling is practised for uniform distribution of medicine and in order to reduce white wood when tree is harvested.
  • On the top portion of the tree where circumference of tree is less than 30 cm  injection recommended approximately for every 25 cm distance in the zig-zag manner.
  • Injection also recommended for the branches and twigs in order to increase the production.

Harvesting of the tree

  • After inoculation resin will be deposited around inoculated place which will be then separated as Chips.
  • Trees can be harvested after 36 months of last round of inoculation.
  • Harvesting of the tree is recommended at about two ft from the ground level and two new suckers are allowed to grow to get next crop.